This thin layer chromatography (TLC) plate comprises a C18-W (Wettable) Silica layer. It can be used in both normal phase or reversed phase separations with eluents from anhydrous solvents to mixtures with high concentrations of water. The relative polarity of the eluent determines the polarity of the layer. The Silica 60 has a surface area of 500 m2/g, mean pore size of 60A, specific pore volume of 0.75 ml/g, particle size of 2-10um, and pH stability of 2-10. The TLC plates contain UV254 fluorescent indicator. The UV-absorbing substances appear as dark-blue to black spots on a light - blue background. This analytical layer is bound to a glass support. It has a thickness of 250um and dimensions of 10x0cm. There are 25 plates per pack. Applications include: aminophenols, barbiturates, preservatives, nucleobases, polycyclic aromatic hydrocarbons, steroids, tetracyclines, plasticizers (phthalates)